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22 January 2014, 08:51 | Updated: 22 January 2014, 09:03
Watch the boys share an ode to their favourite letter of the alphabet in the new clip from the children's TV show.
One Direction can be seen singing an interpretation of their hit single 'What Makes You Beautiful' as they share their love for the letter 'U' on Sesame Street.
Their performance, called 'What Makes U Useful', was uploaded to the children's show's YouTube account this week.
The track starts with the new lyrics: "This letter's 'U'. You know it's true. It starts some words. There are more than a few-e-ew."
The performance follows on from Usher's take on the alphabet during his appearance last year.
Meanwhile, this week it was revealed that One Direction will be releasing two new books in 2014 – including their first ever autobiography.