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It's looking increasingly unlike that there will be a new Panda on it's way in Edinburgh.
Zoo keepers have allowed Sweetie and Sunshine to meet on several occasions but there has been no mating as yet.
The female bear only has a window of 36 every YEAR of fertility.
Experts say that Sweetie's tail was getting in the way.
The Zoo say they have no plans for any artificial insemination at this stage.