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16 March 2013, 09:20 | Updated: 16 March 2013, 10:07
The stars brought back their hugely popular ode to the genre during the singer's final appearance on the show last night.
Justin Timberlake signed off his week-long residency on the Jimmy Fallon show in style by reviving the pair's popular 'History Of Rap' songs.
The pair give a blast through a series of popular rap songs from the past in the series of videos and brought the fourth installment of their collaboration.
Eminem's 'Lose Yourself', Macklemore's 'Thrift Shop' and Nelly are all included as the crowd urged the 'SexyBack' star to take to the mic one more time.
"We can't," Fallon said, with Justin adding: "We couldn't."
Following a bit more teasing with the audience, they launched into the first track – Sugarhill Gang's 'Apache (Jump On It)'.
Watch Justin Timberlake and Jimmy Fallon perform 'The History Of Rap' for the fourth time below:
The appearances have been in the run-up to Justin releasing his new album 'The 20/20 Experience' – which is released on Monday 18th March.